Introduction To run code in parallel by "foreach" and "doParallel" package. Code exmaple library(foreach) library(doParallel) cl
Introduction The processed scRNA data dowloaded from GEO were alwalys like this. Files of multiple samples were deposited together. But
Introduction I found process was shut down due to low memory when I merged multiple seurat objects from different patients.
Introduction Several data are shared in NCBI website, so knowning the methods for downloading data is necessary, especialy for people
Introduction The following are the codes for classifying the samples according to the distance from the centroids of reference class.
Introduction The following are the codes for GO and KEGG enrichment. Code exmaple library(clusterProfiler) library(enrichplot) library(tidyverse) library(magrittr) library(data.table) library(org.Hs.eg.db) setwd(“/home/hxzk/project/XXX/KEGG/”)
Introduction The following are the codes for drawing a volcano plot with ggplot2 Code exmaple #Load package and read file
Tissue CellType Gene Full Name Gene Symbol Reference Epididymal Epithelial cells Principal cell Aquaporin9 Aqp9 [1-3] Epididymal Epithelial cells Principal
Introduction H5ad files were uploaded as available data in some paper of scRNA. But I always analysis scRNA data by
Introduction Here codes from P. Bischoff was showed. It concluded all step in analysis of scRNA, which could be refered